Head of Laboratory

Magdalena Staniszewska, PhD

(staniszkul@kul.pl)

 

The main specialty of the Laboratory of Separation and Spectroscopic Method Applications is developing, validating and applying analytical methods for qualitative and quantitative analyses of biologically active substances including low-molecular, i.e. drugs, metabolites, toxins, pesticides, products of biochemical synthesis and high-molecular compounds, i.e. peptides or proteins using variety of chromatography and mass spectrometry methods. Currently in the Laboratory along with studying the presence of toxin content in food, we carry out the research leading to understanding of disease mechanisms, finding novel therapeutic targets and diagnostics mainly in the field of cancer and metabolic disorders. Our studies performed in collaboration with the Medical University of Lublin and Institute of Immunology and Experimental Therapy Polish Academy of Science in Wroclaw focus on the role of glycation and kynurenine pathway in cancer microenvironment and the receptivity of human endometrium.

            Our interdisciplinary team consists of scientists with competencies in analytical chemistry and biochemistry: Magdalena Staniszewska, PhD,DSc, Anna Stachniuk PhD, Ilona Sadok, MSc, and Ilona Jonik, MSc.
            The Laboratory is equipped with state-of-the-art instruments allowing for sophisticated chromatography and mass spectrometry analysis:

  • Agilent 1290 Infinity Binary liquid chromatograph system coupled with the tandem 6400 Series Triple Quadrupole mass spectrometer (Agilent LC-QQQ6460),
  • Capillary liquid chromatograph/nano-LC (Agilent 1200) provided with the chip-cube valve stator coupled with the tandem 6400 UHD Accurate-Mass Quadrupole Time-of-Flight system (Agilent LC-Q/ToF6538),
  • 1200 Series liquid chromatograph for standard configurations with the new high dynamic range DAD detector anda single quadrupole mass spectrometer (Agilent LC-Q 6120),
  • Agilent Technologies 7890B Gas chromatograph system coupled with the Triple Quadrupole mass spectrometer (Agilent Technologies 7000C)
  • Agilent Technologies 7890B Gas chromatograph system coupled with the mass spectrometer (Agilent Technologies 5977A) equipped with the Head Space (Agilent Technologies 7697A)
  • Analytical 1200 Series liquid chromatograph system with the DAD detector (Agilent 1200)
  • Semi-preparative 1200 Series liquid chromatograph with a UV-VIS detector and a fraction collector (Agilent 1200),
  • MALDI-TOF/TOF Spectrometer (Brucker UltraXtreme #8259900)
  • The Bio-Rad system for protein SDS-PAGE and WB (Bio-Rad MiniProtean Tetra Cell with MiniTrans Blot module)
  • Automatic ASE N350 Accelerated Solvent Extractor system (Thermo Scientific Dionex) the extraction of solid and semisolid samples using common solvents at elevated temperatures and pressures,
  • Automatic Gilson ASPEC GX271 system for the solid phase extraction processing of samples,
  • Mettler Toledo Excellence T50M automatic titrator.

 

Recent papers:

  1. Sławińska A, Fornal E, Radzki W, Skrzypczak K,Zalewska-Korona M, Michalak-Majewska M, Parfieniuk E, Stachniuk A, Study on Vitamin D₂ Stability In Dried Mushrooms During Drying and Storage. Food Chem. 2016, 199: 203-209
  2. Stachniuk, Anna; Fornal Emilia, Liquid Chromatography-Mass Spectrometry in The Analysis of Pesticide Residues in Food, Food Analytical Methods, 2016, 9(6):1654-1665
  3. Fornal Emilia, Stachniuk Anna, Techniki Ekstrakcyjne Stosowane w Oznaczaniu Poozostałości Pestycydów w Żywności Metodą LC-MS, Żywność – Nauka Technologia Jakość 2016, 105(2): 5-18
  4. Greenwald SH, Charette JR, Staniszewska M, Shi LY, Brown SD, Stone L, Liu Q, Hicks WL, Collin GB, Bowl MR, Krebs MP, Nishina PM, Pierce EA, Mouse Models of NMNAT1-Leber Congenital Amaurosis (LCA9) Recapitulate Key Features of the Human Disease, Am J Pathol. 2016, 186(7):1925-1938