Scientific interest and research:

• Overexpression and purification of native or recombinant proteins expressed in E. coli
• Detection and evaluation of enzymatic activities
• Characterization of protein kinase CK2 activities from different organisms
• Estimation of modulating effect of several substances, mainly in regard to kinases
• Research on the mechanism of action in case of inhibitors (interaction inhibitor/enzyme, enzyme/substrate, inhibitor/substrate)

Skills and methods:

• Electrophoresis: proteins (SDS/PAGE, IEF) and nucleic acids (agarose, acrylamide).
• Chromatography techniques (affinity, size-exclusion, ion-exchange, hydrophobic interaction)
• Enzymatic assays to estimate kinase activities