Scientific interest and research:

  • Overexpression and purification of native or recombinant proteins expressed in E. coli
  • Detection and evaluation of enzymatic activities
  • Characterization of protein kinase CK2 activities from different organisms
  • Estimation of modulating effect of several substances, mainly in regard to kinases
  • Research on the mechanism of action in case of inhibitors (interaction inhibitor/enzyme, enzyme/substrate, inhibitor/substrate)

Skills and methods:

  • Electrophoresis: proteins (SDS/PAGE, IEF) and nucleic acids (agarose, acrylamide).
  • Chromatography techniques (affinity, size-exclusion, ion-exchange, hydrophobic interaction)
  • Enzymatic assays to estimate kinase activities