- PhD in medicinal chemistry obtained at University San Pablo CEU (Spain, Madrid) in March 2014.
- Since January 2015 is carrying a 6-month visitant postdoctoral stay at Spanish National Cancer Research Centre (Experimental Therapeutics Programme, Biology Section) in Madrid (Spain).
Scientific interest and research:
Post-translational modifications of proteins, searching of new kinase substrates involved in cancer signalling pathways and angiogenesis, biological evaluation of new drugs, development of dual inhibitors based on interactions between kinases and proteases in cancer, drug design, inhibitors of metalloproteinases, histone deacetylases and protein kinases (CK2), ligands of estrogen receptors.
Skills and methods:
- Microbiological culture
- Cloning and expression of proteins in bacterial and yeast systems
- PCR
- Mutagenesis
- Protein purification (affinity, ion exchange chromatography)
- Evaluation of protein kinase inhibitors (radioisotopic method)
- Identification of new kinase substrates
- Evaluation of protein-protein interactions (immunoprecipitation)
- Gel electrophoresis (SDS-PAGE, zymography), Western Blotting
- Radioligand Binding Assays
- Surface Plasmon Resonance Technology (Biacore T100)
- Metalloproteinase and histone deacetylase inhibition assays (colorimetric, fluorimetric, luminometric)
- Cell culture (normal and cancer-origin human cell lines)
- Cell based assays (cell viability, proliferation, migration, invasion, reporter gene assays, drug permeability (Caco-2))
