Scientific interest and research:
- Overexpression and purification of native or recombinant proteins expressed in E. coli
- Detection and evaluation of enzymatic activities
- Characterization of protein kinase CK2 activities from different organisms
- Estimation of modulating effect of several substances, mainly in regard to kinases
- Research on the mechanism of action in case of inhibitors (interaction inhibitor/enzyme, enzyme/substrate, inhibitor/substrate)
Skills and methods:
- Electrophoresis: proteins (SDS/PAGE, IEF) and nucleic acids (agarose, acrylamide).
- Chromatography techniques (affinity, size-exclusion, ion-exchange, hydrophobic interaction)
- Enzymatic assays to estimate kinase activities